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MC/CAR(人外周血多发性骨髓瘤细胞)

CBP60821

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I. General information 
Synonyms: MC/CAR
Background: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.
Species: Homo sapiens, human
Tissue: peripheral blood
Disease: plasmacytoma; myeloma
Gender: male, 81 years,  Caucasian
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 11,14
D7S820: 9,13
THO1: 6,9
TPOX: 8
vWA: 17,18
Cobioer’s Cell Line Authentication Service
Culture Medium:

IMDM+20%FBS

MC/CAR完全培养基,# CBP60821M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
Receptor Expression: complement (C3b)
Genes Expressed: immunoglobulin
Cellular Products: immunoglobulin
Tumor Formation: Yes, in nude mice inoculated subcutaneously with 107 cells
(Tumors developed within 21 days at 100% frequency (5/5).)
Comments: The cells produce, but do not secrete, immunoglobulin.
No surface immunoglobulin.
8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
For more information, please contact Cobioer (4008-750-250).

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