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MOLM-13(人急性髓细胞样白血病)

CBP60678

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I. General information 
Synonyms: MOLM-13
Background: established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14
Species: human (Homo sapiens)
Tissue: N/A 
Disease: acute myeloid leukemia
Gender: N/A 
Morphology: N/A
Growth Mode: Suspension
Doubling Time: ca. 50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI1640+20%FBS

MOLM-13完全培养基,#CBP60678M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -; 
Fingerprint: multiplex PCR of minisatellite markers revealed a DNA profile identical to MOLM-14
Species: confirmed as human by PCR speciation and cytogenetics
Cytogenetics: human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
 
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