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GOS-3(人胶质细胞细胞)

CBP60784

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I. General information 
Synonyms: GOS-3
Background: originally described as a glioma cell line established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); however, DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG; described in the literature to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation
Species: Homo sapiens, human
Tissue: brain
Disease: mixed astro-oligodendroglioma
Gender: famale,55 years
Morphology: glioblastoma 
Growth Mode: fibroblastoid, adherent cells growing as monolayer
Doubling Time: 50 hours
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 7
TPOX: 9,11
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+10%FBS+4 mM L-glutamine

GOS-3完全培养基,# CBP60784M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Comments:

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays     

Cytogenetics: human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 typical in low-grade astrocytoma-oligodendroglioma - gains of sex chromosomes (particularly Y) are very rare in tumor cells
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin + Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
For more information, please contact Cobioer (4008-750-250).

药靶模型联系方式: 华东销售经理:18240630236 全国销售经理:18066071954
诊断标准品联系方式: 华东销售经理:15000320447 华北销售经理:18131625521 华南销售经理:13484295986 华中&西南销售经理:13871580511 全国销售经理:13484295986

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