Sp2/0-Ag14（小鼠骨髓瘤细胞）
CBP60824
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| I. General information | |
| Synonyms: | Sp2/0-Ag14 |
| Background: | SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 µg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies |
| Species: | murine (mouse) (Mus musculus) |
| Tissue: | N/A |
| Disease: | myeloma |
| Gender: | N/A |
| Morphology: | single, round cells in suspension |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30-40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS+1mM Sodium Pyruvate Sp2/0-Ag14完全培养基，# CBP60824M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | N/A |
| Fingerprint: | N/A |
| Species: | Mus musculus origin confirmed by COI DNA barcoding |
| Cytogenetics: | N/A |
| Virus | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
