AML12（小鼠正常肝细胞）
CBP60909
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| I. General information | |
| Synonyms: | AML12 |
| Background: | The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha. |
| Species: | Mus musculus, mouse |
| Tissue: | liver |
| Disease: | normal |
| Gender: | male, 3 months |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 37 hrs |
| Culture Medium: |
DMEM/F12(1:1)+10%FBS+ITS(GIBCO 41400045)+ Dexamethasone 40 ng/ml AML12完全培养基，# CBP60909M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha. |
| Cellular Products: | albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha. |
| Tumor Formation: | No, the cells do not form colonies or grow in soft agar. No, the cells were not tumorigenic in immunosuppressed mice |
| Comments: | The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha. By electron microscopy, these cells exhibit typical hepatocyte features such as peroxisomes and bile canalicular like structure. AML12 cells retain the capacity to express high levels of mRNA for serum (albumin, alpha 1 antitrypsin and transferrin) and gap junction (connexins 26 and 32) proteins, and contain solely isoenzyme 5 of lactate dehydrogenase. The cells express high levels of human TGF alpha and lower levels of mouse TGF alpha. Expression of liver specific proteins decreases with time in culture, but is reactivated by growing the cells in serum free medium. For more information, please contact Cobioer (4008-750-250). |
