BFTC-905（人膀胱移行细胞癌）
CBP60638
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| I. General information | |
| Synonyms: | BFTC-905 |
| Background: | established from a 51-year-old Chinese woman with a grade III papillary transitional cell carinoma of the urinary bladder in 1990; patient lived in a blackfoot disease endemic area in Taiwan |
| Species: | human (Homo sapiens) |
| Tissue: | urinary bladder |
| Disease: | urinary bladder transitional cell carcinoma |
| Gender: | N/A |
| Morphology: | epitheloid cells growing adherently in monolayers, forming large islets; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 60-70 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+15%FBS BFTC-905完全培养基，# CBP60638M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays; bacterial contamination was eliminated with Baytril (enrofloxacin) and BM-Cyclin (tiamulin & minocycline) |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+) |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH |
| Cytogenetics: | human flat-moded hyperdiploid/hypotriploid karyotype - 58(52-61)<3n>X/XX-X, -4, +5, -9, -13, -15, -16, -18, -21, -22, -22, +mar, add(1)(p36), i(5p), add(5)(q35), del(9)(p22), del(11)(p11), add(11)(q13), add(13)(p12), add(13)(p12), add(15)(p12), add(19)(p13) - i(5p) and loss of 9p associated with transitional cell carcinoma - matches published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
