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M059J(人脑恶性胶质母细胞瘤)

CBP60596

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I. General information 
Synonyms: M059J
Background: M059J cells were isolated from a tumor specimen taken from a 33 year old male with untreated malignant glioblastoma,The cells were isolated concurrently from the same tumor specimen as M059K (see CRL-2365).
Species: Homo sapiens, human
Tissue: brain
Disease: malignant glioblastoma; glioma
Gender: male, 33 years
Morphology: fibroblast
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 14
D16S539: 10,12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM/F12 + 10% FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

M059J完全培养基,# CBP60596M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Comments: M059J cells lack DNA-dependent protein kinase activity, while M059K cells express normal levels of DNA-dependent protein kinase. M059J cells are approximately 30-fold more sensitive to ionizing radiation than M059K cells.
M059J cells are more sensitive than M059K cells to the cytotoxic effects of bleomycin, N,N-bis(2-choroethyl)-N-nitrosourea and nitrogen mustard. M059J cells are deficient in repair of DNA double strand breaks.
The cells are negative for glial fibrillary acidic protein (GFAP).
For more information, please contact Cobioer (4008-750-250).

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