MOLP-8（人多发性骨髓瘤）
CBP60562
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| I. General information | |
| Synonyms: | MOLP-8 |
| Background: | established from the peripheral blood of a 52-year-old Japanese man with multiple myeloma (stage IIIA, type IgD lambda) in 2002 |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | multiple myeloma |
| Gender: | N/A |
| Morphology: | single round cells in suspension, some cells loosely adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+20%FBS MOLP-8 完全培养基，# CBP60562M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, smlambda -, cylambda +; |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics and species PCR |
| Cytogenetics: | human flat-moded near-diploid karyotype - 40-48<2n>X, -Y, -15, +1-2mar, t(3;8)(p22;q22), der(4)t(4;15)(q32;q21), inv(8)(q21q24), der(11)t(?4 ;11)(q31;q13), t(11;14)(q13;q32), t(16;18)(p13;q21), der(17)t(11;17)(q13;p13) - carries t(11;14) effecting IGH-CCND1 juxtaposition with additional MYC rearrangement present in inv(8) - matches published karyotype |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
