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RWPE-1(人前列腺上皮细胞)

CBP60347

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I. General information 
Synonyms: RWPE-1
Background: Epithelial cells derived from the peripheral zone of a histologically normal adult human prostate were transfected with a single copy of the human papilloma virus 18 (HPV-18) to establish the RWPE-1 (ATCC CRL-11609) cell line. Ref
Species: Homo sapiens, human
Tissue: prostate
Disease: normal
Gender: male, 54 years adult, Caucasian, White
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14,18
Cobioer’s Cell Line Authentication Service
Culture Medium:

Keratinocyte Serum Free Medium (K-SFM), Kit  (GIBCO Catalog Number 17005-042)

RWPE-1完全培养基,# CBP60347M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen),Ref (upon exposure to androgen)
Receptor Expression: androgen receptor, expressed , Ref (upregulated upon exposure to androgen)
Genes Expressed: cytokeratin 18, cytokeratin 8,  Tumor Supressor Gene(s): p53 +, pRB + Ref
Cellular Products: cytokeratin 18   cytokeratin 8
Tumor Formation: No, in nude mice (with or without Matrigel)  Ref      No, in soft agar  Ref
Comments: In 3-dimensional Matrigel culture, RWPE-1 cells organize into acini and secrete PSA into the lumen when exposed to androgen. Ref
When injected with Matrigel or with stromal cells, into male athymic rodents, RWPE-1 cells also organize into acini Ref and produce PSA.
Cells from the RWPE-1 cell line were further transformed by Ki-ras using the Kirstin murine sarcoma virus (Ki-MuSV) to establish the tumorigenic RWPE-2 cell line (ATCC CRL-11610) Ref and the RWPE2-W99 (ATCC CRL-2853) cell line.
Further, a family of tumorigenic cell lines, that mimics multiple steps in prostate cancer progression, was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrosourea (MNU). See the WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell lines.
The depositor reports that the RWPE-1 cell line (ATCC CRL-11609) was screened, and found negative for, Hepatitis B virus, Hepatitis C virus and Human immunodeficiency virus. ATCC confirmed this cell line is positive for the presence of HPV viral DNA sequences via PCR.
For more information, please contact Cobioer (4008-750-250).

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