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VPAC1/CRE-Luc/HEK293

CBP71385

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I. Background
The VPAC1 receptor which is an archetype of the class B GPCRs binds Vasoactive Intestinal Peptide (VIP), a neuropeptide widely distributed in central and peripheral nervous system modulating many physiological processes including regulation of exocrine secretions, hormone release, foetal development, immune response … VIP appears to exert beneficial effect in neurodegenerative and inflammatory diseases.
 
II. Introduction
Host Cell:

HEK293

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+2ug/ml puromycin+200ug/ml hygromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Transducer: Gs
 
Ⅲ. Description of Host Cell Line
Organism: Human
Tissue: kidney
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. Recombinant VPAC1/CRE-Luc/HEK293 stably expressing human VPAC1.

Figure 2. Dose response of Agonists in VPAC1 CRE-Luc HEK293 Cells (Clone13).


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