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IL12 Effector Reporter Cell

CBP74134

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I. Background
Interleukin 12 (IL-12) family is unique in having the only heterodimeric cytokines, including IL-12, IL-23, IL-27 and IL-35. The heterodimeric cytokines of the IL-12 family consist of an α-chain (p19, p28 or p35) and a β-chain (p40 or Ebi3). 
 
II. Introduction
Expressed gene: IL12R
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM +10%FBS+2ug/ml puromycin+200ug/ml hygromycin+5ug/ml blasticidin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Representative Data

Figure 1. Dose Response of Recombinant Human IL12 in Human IL12 Effector Reporter Cells (C2).

Figure 2. Inhibition of IL12-induced Reporter Activity by IL-12/IL -23 p40 Neutralizing Antibody in IL12 Effector Reporter Cells (Clone2).

 

 


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