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IL23 Effector Reporter Cell

CBP74130

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I. Background
IL-23 is a member of the IL-12 cytokine family, which also includes members such as IL-12, IL-27, IL-35 and IL-39, which are a unique set of heterodimeric cytokines, It usually consists of an alpha chain subunit (p19, p28, or p35) and a beta chain subunit (p40 or Epstein-Barr virus-induced gene 3), and signals are transduced through the JAK/STAT pathway.
 
II.Introduction
Expressed gene: IL23R
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): IL-23 Receptor protein, IL-23R protein;Interleukin-23 receptor, IL23R, IL-23 receptor, IL-23R
Freeze Medium: 90% FBS+10%DMSO
Culture Medium: DMEM +10%FBS+2ug/ml puromycin+200ug/ml hygromycin+5ug/ml blasticidin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
 
III. Representative Data

Figure 1. Recombinant IL23 Effector Reporter Cell stably expressing IL23R.

Figure 2. Recombinant IL23 Effector Reporter Cell stably expressing IL12β1.

Figure 3.Dose Response of Recombinant Human IL23 in Human IL23 Effector Reporter Cells (C6).

Figure 4.Inhibition of lL23-induced Reporter Activity by IL23 Neutralizing Antibody in IL23 Effector Reporter Cells (Clone6).

 

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